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課程名稱 |
質譜技術應用
Application of Mass Spectrometry |
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開課學期 |
110-2 |
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授課對象 |
公共衛生學院 食品安全與健康研究所 |
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授課教師 |
羅宇軒 |
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課號 |
IFSH7019 |
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課程識別碼 |
851EM1190 |
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班次 |
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學分 |
2.0 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
星期一3,4(10:20~12:10) |
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上課地點 |
公衛211 |
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備註 |
本課程以英語授課。
總人數上限：35人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
This course seeks to introduce students to the fundamentals and applications in mass spectrometry, from sample preparation, separation technique such as chromatography, common ionization sources, mass analyzers to chemical databases. Students will be able to devise and develop appropriate analytical methods using mass spectrometry for known (quantitative), suspect screening, or untargeted analyses. Students will be able to apply these toolkits to solve the environmental and food safety issues in real-life.
本課程旨在向學生介紹質譜分析技術的理論基礎與應用，從樣本前處理、層析分離技術、常用游離源、質量分析器到化學資料庫等，使學生具備設計、發展合適的質譜分析方法以進行已知物(定量)、疑似物比對或未知物分析，以解決實際生活中會遇到的環境/食品安全議題。
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課程目標 |
At the end of the course the students should be able to
1. Recognize and identify the potential hazards existing in the food supply chain and manufacturing processes (MS-B)
2. Equip with the fundamentals and research ability in mass spectrometry (choose, devise, and develop an analytical MS method to answer the real-life questions).
3. Critically evaluate studies using mass spectrometry and understand the underlying limitations
在課程結束時，學生應能
1. 認知與偵測危害在食品供應鏈與製程中可能出現之處，並能進行危害分析與風險控管
2. 具備質譜分析的基本知識與研究能力(如何選擇、設計、並發展合適的質譜分析方法以回答現實生活中的問題)
3. 批判性評估質譜相關文獻並了解其潛在的研究限制
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課程要求 |
Midterm and final exam; Group or individual projects; in-class discussion |
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預期每週課後學習時數 |
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Office Hours |
另約時間 備註： By appointment |
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指定閱讀 |
Mass Spectrometry: Principles and Applications 3rd Ed., Edmond de Hoffmann and Vincent Stroobant, John Wiley & Sons Ltd, 2007 (醫學圖書館 索書號: QD96.M3 H6413 2007, 條碼: 3788446) |
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參考書目 |
1. Mass Spectrometry: A Textbook, Gross, JU+FCrgen H, Springer, 2017 (e-book available)
2. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis, Mu Naushad, Mohammad Rizwan Khan, Taylor & Francis Group, LLC, 2014 (e-book available)
3. Practical Gas Chromatography: A Comprehensive Reference, Katja Dettmer-Wilde and Werner Engewald, by Springer-Verlag Berlin Heidelberg, 2014 (e-book available)
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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Week 1 |
2/14 |
History, basic concepts and terminology of mass spectrometry
質譜的歷史、基本觀念與名詞定義 |
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Week 2 |
2/21 |
Sample preparation and derivatization
樣本前處理與衍生化 |
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Week 3 |
2/28 |
228 Peace Memorial Day (National Holiday)
二二八和平紀念日 |
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Week 4 |
3/7 |
Separation technique I: Liquid chromatography
分離技術I:液相層析 |
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Week 5 |
3/14 |
Separation technique II: Gas chromatography and ion mobility spectrometry
分離技術II: 氣相層析與離子遷移譜 |
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Week 6 |
3/21 |
Sample Ionization I: EI, CI, APCI, and APPI
樣本游離技術I |
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Week 7 |
3/28 |
Sample Ionization II: ESI, ICP, FAB, SIMS, and MALDI
樣本游離技術II |
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Week 8 |
4/4 |
Children’s Day (National Holiday)
兒童節 |
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Week 9 |
4/11 |
Midterm exam
期中考 |
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Week 10 |
4/18 |
Mass analyzer I
質譜分析器I |
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Week 11 |
4/25 |
Mass analyzer II
質譜分析器II |
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Week 12 |
5/2 |
Quantitative analysis: Method validation & quality assurance/ quality control
定量分析:方法開發與確效、品管 |
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Week 13 |
5/9 |
Nontargeted analysis and suspect screening: database, tools, and application
未知物分析與疑似物比對:資料庫、工具以及相關應用 |
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Week 14 |
5/16 |
Group projects of Special Topics (I)
小組質譜文獻報告 |
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Week 15 |
5/23 |
Group projects of Special Topics (II)
小組質譜文獻報告 |
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Week 16 |
5/30 |
Final exam
期末考 |
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